enzymes

Tom Chappell dmcbtch at ucl.ac.uk
Wed Jul 19 11:12:10 EST 1995


> Subject: enzymes
> From:    dowdeswm at meena.cc.uregina.ca at internet
> Date:    7/14/95  22:54
> 
> I need a specific example of an enzyme that will denature and undergo
> renaturation.  If anyone can help please provide the name of the enzyme,
> where it is found and what it does, what conditions cause denaturation,
> and what conditions cause renaturation.  Also please specify if the
> renaturation can occur when the enzyme is isolated in a test tube, or if
> chaperones are needed to obtain the correct tertiary structure.
> 
> Thanks for any help you can provide.
> 
> Marla Dowdeswell
> University of Regina, Saskatchewan
> 
There is, of course, that classic known to all molecular biologists:
RNase.

Denaturation: boiling
Renaturation: virtually anything except boiling...



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