enzymes

Mays, Peter PMays at organo.com
Wed Jul 19 10:28:48 EST 1995


One enzyme which I think meets your requirements is lysyl oxidase (protein 
lysine 6-oxidase, EC 1.4.3.13), its a secreted enzyme which is responsible for 
initiating crosslinking in collagen and elastin by oxidizing specific lysine 
residues.  This enzyme is recoverable from 8M urea, and is active in a test 
tube.

For further information see:

J Biol Chem 1992, 267, 8666

Methods in Enzymology 1982, 82, 305-319

I hope this may be some use.

Peter Mays
Organogenesis Inc
150 Dan Road
Canton MA 02021
_______________________________________________________________________________
Subject: enzymes
From:    dowdeswm at meena.cc.uregina.ca at internet
Date:    7/14/95  22:54

I need a specific example of an enzyme that will denature and undergo
renaturation.  If anyone can help please provide the name of the enzyme,
where it is found and what it does, what conditions cause denaturation,
and what conditions cause renaturation.  Also please specify if the
renaturation can occur when the enzyme is isolated in a test tube, or if
chaperones are needed to obtain the correct tertiary structure.

Thanks for any help you can provide.

Marla Dowdeswell
University of Regina, Saskatchewan


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