GFP expression in mammalian cells

Tom Chappell dmcbtch at ucl.ac.uk
Wed Jul 19 11:09:02 EST 1995


In article <3uj93k$2t8 at cwis-20.wayne.edu>
Anton Scott Goustin <asg at cmb.biosci.wayne.edu> writes:

> Question for Dr. Nicoletti:
> I am interested in using GFP in the context of flow cytometry.  The 
> standard excitation source misses the absorbance optimum for GFP.  Do 
> you have any experience here?


the original GFP clone works well (great) with the FITC excitation line
of the laser on the BioRad confocal microscope. This is well off the
excitation optimum. Flow cytometry lasers should be using the same
laser line.

Tom Chappell
MRC Lab for Mol. Cell Biology



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