Protein conc from A280 & sequence

[A.S.McAlpine]

In article <3upe58$ko4 at ixnews7.ix.netcom.com>, mesalvu at ix.netcom.com (Michael Salvucci) writes:
|> 
|> Does anyone know of a method (or computer program) for accurately
|> determining the concentration of a protein from the A280 and the
|> sequence (i.e., the number of tyr & trp residues).
|> 
|> Thanks in advance,
|> 
|> Mike Salvucci, USDA-ARS
|> Phoenix, AZ
|> 
|> msalvucc at asrr.arsusda.gov

Have a look at the following

		Gill & von Hippel
		Anal. Biochem. 182 pp 319-326 (1989)

this should be of help




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