Co-Immunoprecipitations

Ron Goto x2832 rgoto at smtplink.coh.org
Fri Jul 28 10:19:51 EST 1995


In article <kalch-2307951827550001 at hayci.generes.ca> kalch at ulam.generes.ca (Mikek) writes:

Kalch

Yes it is heavy and light chain you see on your gel. (so if your cold 
co-precipitate is of similiar size). #2  what I'm not sure of is your HEK293 
cell lysate also in 1% NP-40, if it is then you are in micelle, and I don't 
know enough about  detergent chemistry, critical micelle concentration and 
etc to comment about co-precipitation. Just a suggestion but can you crosslink 
before you precipitate? (does your Ab still bind the protein?)

good luck
Ronald



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