Dissolvable IEF gels?

Donna O. Perdue dperdue at wolfe.net
Mon Jul 31 14:50:12 EST 1995


Does anyone know of a method for making polyacrylamide IEF gels whose 
crosslinks can be disrupted later?  I know there are some protocols for 
dissolvable SDS-PAGE gels, but what about IEF? 

 I am currently using a native IEF protocol to analyze cell wall enzymes in 
ripening fruit, and would like to recover proteins of interest.  We have an 
electroeluter,  but I was wondering about other methods 
for recovering proteins from nondenaturing polyacrylamide IEF gels.  

Thanks in advance.

D.O. Perdue
USDA/ARS



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