HIS-tag proteins

Tara McCready tara_mccready at darwin.biochem.ualberta.ca
Mon Jul 31 10:00:20 EST 1995


In article <3v3k25$t8u at sifon.cc.mcgill.ca>, anne-claude
<gingras at medcor.mcgill.ca> wrote:

> Hi,
> 
> I am having a tough time trying to purify his-tagged proteins using 
> QUIAGEN system. 

There has been quite a lot of discussion about this in the
'bionet.molbio.methds-reagnts' newsgroup.  Check out the recent TIBS
article (July95, vol20 pp285-6) which deals with some of the problems you
mentioned.

Tara McCready
Dept Biochemistry
Univ of Alberta



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