Protein conc from A280 & sequence

Peter McPhie pmcphie at helix.nih.gov
Mon Jul 31 15:53:33 EST 1995


This procedure is not too reliable because od the contributions from 
other side chains, notably cys-cys. But try Prasad et al (1986) Bichem. 
25, 5214, using A280 and A215.
				P.M.


On 21 Jul 1995, Michael Salvucci wrote:

> 
> Does anyone know of a method (or computer program) for accurately
> determining the concentration of a protein from the A280 and the
> sequence (i.e., the number of tyr & trp residues).
> 
> Thanks in advance,
> 
> Mike Salvucci, USDA-ARS
> Phoenix, AZ
> 
> msalvucc at asrr.arsusda.gov
> 
> 



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