Immunobloting of Albumin...

[Paulo Barros Alexandrino]

: Dear All
: I would like to know what kind of blocking agent would be advisable
: to employ for immunological probing of albumin after transfering the
: protein to a nitrocellulose matrix. Use of gelatin or non-fat milk
: is yelding a too undesirable background.
: Thanking in advance any answers...
: Paulo Alexandrino
: University of Porto, PORTUGAL
: E-mail: pbalexan at fc1.fc.up.pt