serum protein delipidation question
dmb618 at enh.nist.gov
Tue Mar 14 16:01:06 EST 1995
Does anyone have any "tried-and-true" methods (or
references to methods) for defatting serum proteins?
I've been trying to isolate albumin from serum on a
microscale using antibody affinity chromatography.
While I have been able to extract the albumin, the
albumin retains its complement of bound small
lipophilic molecules (which interfere with the
quantitation I ultimately want to do).
I have tried to defat the extracted albumin using the
standard free fatty acid extraction solvent of
chloroform/MeOH (2:1). The problem here is that the
MeOH precipitates the protein which settles on the
chloroform-water interface, making it difficult to
Does anyone have any suggestions?
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