serum protein delipidation question
Wayne R. Baker
baker at iastate.edu
Wed Mar 15 21:38:38 EST 1995
In bionet.molbio.proteins, D.M. Bunk <dmb618 at enh.nist.gov> wrote
:Does anyone have any "tried-and-true" methods (or
:references to methods) for defatting serum proteins?
I have one that works for us and our collaborators (who developed it). We
have a lipid-binding protein and we need to get rid of any heterogeneous
lipids that are bound during culture. After purification, the protein is
passed over a hydroxyalkoxypropyl dextran column. The resin is available
from Sigma. Take a look at Xu et al. (1993) J. Biol. Chem. vol. 268
p7874 and Beijer & Nystrom (1972) Anal. Biochem. vol 48 p1.
Drop me an e-mail if you want the procedure we use.
Wayne Baker (baker at iastate.edu) Few things are harder to put up with
4288 Molecular Biology Building than the annoyance of a good example.
Iowa State University -Mark Twain
Ames IA 50011
(515) 294 0781
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