Molar extinction coefficient of a protein at 280 nm.

David S. Gottfried gottfrie at
Thu Mar 16 12:16:24 EST 1995

In article <3k9cps$nhe at>
madi at (Marc DIEU) writes:

> Is it possible to determine the molar extinction coefficient at 280 nm
> of a given protein (epsilon 280 nm prot.) simply by additionning the
> individual molar extinction coefficients of the different aromatic
> residues which compose the sequence of the protein ? Is it correct or
> not ? Is there any literature available about it ?

Check out "Calculation of protein extinction coefficients from amino
acid sequence data", S. C. Gill and P. H. von Hippel, Analytical
Biochemistry, 182, 319-326 (1989).

Good Luck

David S. Gottfried
Department of Physiology and Biophysics
Albert Einstein College of Medicine

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