antibody compatibility

Fiberman william at neuro.usc.edu
Thu Mar 16 19:03:28 EST 1995


I want to do immunocytochmistry with sections of rabbit brains.  What would
be a bigger problem:  if the primary antibody is generated in rabbits or
if the secondary antibody is generated in rabbits?  Assuming both are 
polyclonal antibodies.  Are there tricks to reduce background if one of
the antibodies is from rabbit?

-fm




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