antibody compatibility

[Shahram Mori]

Fiberman (william at neuro.usc.edu) wrote:
: I want to do immunocytochmistry with sections of rabbit brains.  What would
: be a bigger problem:  if the primary antibody is generated in rabbits or
: if the secondary antibody is generated in rabbits?  Assuming both are 
: polyclonal antibodies.  Are there tricks to reduce background if one of
: the antibodies is from rabbit?

: -fm

I would try affinity Purifying my antibodies using sephadex columns.
Cheers
--
Shahram Mori					   _/\_
Program in Molecular Biology			  _\  /_
Dept. of chemistry and Biochemistry Box 3C	  \_  _/
NMSU  Las Cruces NM				    ||
88003