Solublizing nasty peptide

Rick Neubig RNeubig at umich.edu
Mon Mar 20 16:23:10 EST 1995


Hello netters,

I have been trying to solublize a particularly nasty 33-mer 
peptide which is moderately hydrophobic.

I can get it into DMSO or acetonitrile but it forms an opalescent
suspension when I try to dilute into aqueous solutions (at either
1 mM or slightly less). Any suggestions about how to get it into 
solution with some semblence of a "native" conformation - 
which is the aim of the work.

Thanks in advance,
Rick Neubig
http://www.umich.edu/~rneubig



More information about the Proteins mailing list