Desalting small basic proteins on Sephadex G-10

[Mark W. Trumbore]

In article <1AF864B762F at botany.uq.oz.au>, J.Marcus at botany.uq.oz.au
("MARCUS, DR J.") wrote:


>     Has anyone ever tried ethanolamine as a volatile buffer 
> to use in conjuction with deslating on Sephadex G-10?  Would it be a 
> problem for the vaccuum pumps?  Thanks in advance for any 
> help.  
> 
> Sincerely,
> John Marcus

I have not used ethanolamine but do use triethylamine regularly.  It is
volatile and has not harmed my vacuum pump.  It is also much easier (in my
opinion) to work with than ethanolamine.

Mark Trumbore

-- 
Mark W. Trumbore
National Institutes of Health
National Cancer Institute
Building 37, Room B109
Bethesda, MD 20892
Phone: (301)496-6682
Fax:   (301)402-0213
Email: Trumborm at dc37a.nci.nih.gov