phage display
kend
kend
Tue Mar 21 09:32:58 EST 1995
I've heard a little bit about a new technique that sounds very
promising called phage display. From what I understand you insert a library
of DNA into a phage in such a way that the protein coded for by the DNA insert
is "displayed" on the surface of the phage coat. Then you could immobilize
your protein of interest (using GST or the like) and pull down not only the
proteins which interact with yours but the DNA that codes for the protein as
well.
I'd like to try this in my experements but would like to know more
about it and discuss it with others who have experience with the technique
or at least have heard about it.
Ken Davenport
kend at owlnet.rice.edu
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