Molar extinction coefficient of a protein at 280 nm.

PL at jlollar.wmb.emory.edu PL at jlollar.wmb.emory.edu
Tue Mar 21 22:10:59 EST 1995



> > Perhaps a more rigorous way to get an ext. coefficient at 280 nm is to use 
thesimulataneous measurement at 205 nm and the fact the peptide bonds 
contribute most of the absorbance at 205 nm and the ext. coeff. of a peptide 
bond is invariant.  Check out van Iersal et al. Anal. Biochem. 151, 196-204, 
1985.  I have used this method with success, although it is not painless.





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