native PAGE gels
ojar.melefors at postmac.cmb.ki.se
Wed Mar 22 12:15:28 EST 1995
In article <3jfgru$ddm at susscsc1.rdg.ac.uk>, abskhuri at reading.ac.uk (S.
Does anyone out there have any information about native PAGE gels?
The books only seem to skim over the subject, are there any Idiot's Guides
out there that I could have a look at?
I used native PAGE with subsequent staining for enzyme activity within the
gel, e.g. for plant peroxidases (P. Jordan et al.,1990 Electrophoresis 11,
653-655) or for human alkaline phosphatase (P. Jordan et al.,1992 Biochem.
Int. 26, 97-104). You might wish to check the protocols. Nothing special:
just the Laemmli-system without SDS, sample loading using sucrose, running
buffer a Tris/glycine system. (Let me know if you need more information
under e-mail hpeter at fm.ul.pt)
More information about the Proteins