Silver staining of proteins on PVDF?

Rick Neubig RNeubig at umich.edu
Wed Mar 22 17:10:24 EST 1995


mcroos at imb-jena.de (Martin Roos) wrote:

> does anybody have suggestions/literature, how to "silver-stain" proteins
> after the transfer to PVDF-membranes?

Colloidal gold works great on PVDF! It is probably as sensitive as 
silver staining gels but actually seems cleaner to us.

We use a kit from BioRad.

Rick Neubig
http://www.umich.edu/~rneubig




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