storing western blots

Gerard gjmadlam at umich.edu
Wed Mar 22 11:42:24 EST 1995


In article , william at neuro.usc.edu (Fiberman) wrote:

> Dear Netters,
> 
> After I run the SDS-PAGE and transfer proteins onto nitrocellulose, what is
> the best way to store the filter to minimize protein degradation?  Can I
> just let it dry up?
> 
> -fm

Hi,

I've been freezing my blots (in KAPAC pouches) after the transfer
procedure.  This seems to work well.  I've even been able to develop my
blots, using ECL, freeze them, and then re-develop them without any loss
in signal (protein).  Hope this helps.

Gerard



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