Solublizing nasty peptide

cheethj at ROCKVAX.ROCKEFELLER.EDU cheethj at ROCKVAX.ROCKEFELLER.EDU
Wed Mar 22 10:04:26 EST 1995


>I have been trying to solublize a particularly nasty 33-mer 
>peptide which is moderately hydrophobic.
>

Do you think that this hydrophobic peptide interacts with membranes?

If it is, you could try drying it down from organic solution in the
presence of lipid, then resuspending in aqeous buffer.

>I can get it into DMSO or acetonitrile but it forms an opalescent
>suspension when I try to dilute into aqueous solutions (at either
>1 mM or slightly less). Any suggestions about how to get it into 
>solution with some semblence of a "native" conformation - 
>which is the aim of the work.
>
>Thanks in advance,
>Rick Neubig
>http://www.umich.edu/~rneubig
>
>
>

_______________________________________
Dr. James J. Cheetham
Laboratory of Molecular 
and Cellular Neuroscience
The Rockefeller University
1230 York Avenue
New York, NY 10021
(212) 327-8786
cheethj at rockvax.rockefeller.edu
_______________________________________




More information about the Proteins mailing list