Slowly migrating EGF receptor

Dayanidhi Raman maraman at
Sun Mar 26 17:36:43 EST 1995

Hi, I immunoadsorbed the EGF receptor and eluted it, dialyzed to remove the detergent in the presence of added lipid, assayed for tyrosine kinase activity. The sample was heated to 60 C for 3 min. in laemmli's sample buffer and was run on  7.5 % SDS-PAGE. The autoradiogram showed all the active receptors at the very   top of the gel. Need help quickly!

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