Nonidet P-40

Nick Fisher nef at mole.bio.cam.ac.uk
Mon Mar 27 05:50:49 EST 1995


In article <baxley-2603951452030001 at fp1-molbio-13.uoregon.edu>,
baxley at molbio.uoregon.edu (Lara Endres Baxley) wrote:

> Does anyone know what Nonidet P-40 is for and where I can get it?  It's in
> a purification protocol which calls for 0.01% Nonidet P-40 in all of the
> buffers.
 

Nonidet P-40 is a non-ionic detergent (nonaethylene glycol octylphenyl ether)
with a cmc around 0.2 mM in 50 mM salt (so it's going to be tricky to remove
by dialysis). Its CAS registry no. is 9036-19-5. Try getting some from
Sigma or Calbiochem.

Nick Fisher
Biochemistry Dept. 
Cambridge University
England.



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