oxidation of SS bridges

Christopher Walentas Bioc cdw1000 at mole.bio.cam.ac.uk
Tue Mar 28 03:43:22 EST 1995


Hi.

I was wondering if anyone out there would share their favorite ways
for oxidising disulphide bridges.  I've engineered in a couple into
my enzyme, but they don't seem to want to form.  Making the grand
assumption that they ever will, how should I progress?  Folk have
mentioned CuSO4, K3Fe(CN)6 and iodosobenzoic acid, but I need to
chase down some refs. for concns, pH, etc...  ANY and all help on
the subject would be appreciated.  

Cheers in advance!

Tripp

-------------------------------------------------------------------------------
C. D. Walentas
Cambridge Centre for Molecular Recognition,
University of Cambridge,
Cambridge, England CB2 1QW

Phone:  0223 333 662
Fax:    0223 333 345
email:  c.d.walentas at bioc.cam.ac.uk




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