oxidation of SS bridges

Christopher Walentas Bioc cdw1000 at mole.bio.cam.ac.uk
Tue Mar 28 03:43:22 EST 1995


I was wondering if anyone out there would share their favorite ways
for oxidising disulphide bridges.  I've engineered in a couple into
my enzyme, but they don't seem to want to form.  Making the grand
assumption that they ever will, how should I progress?  Folk have
mentioned CuSO4, K3Fe(CN)6 and iodosobenzoic acid, but I need to
chase down some refs. for concns, pH, etc...  ANY and all help on
the subject would be appreciated.  

Cheers in advance!


C. D. Walentas
Cambridge Centre for Molecular Recognition,
University of Cambridge,
Cambridge, England CB2 1QW

Phone:  0223 333 662
Fax:    0223 333 345
email:  c.d.walentas at bioc.cam.ac.uk

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