Cyanogen Bromide Digest Question

D.M. Bunk dmb618 at
Fri Mar 31 10:20:32 EST 1995

I am planning on doing a cyanogen bromide digest of 
huamn serum albumin next week and I was looking for 
some pointers from anyone with CNBr digest experience.

1.	The method I found calls for dissolving the 
protein in 70% formic acid, adding solid CNBr (2 mg 
CNBr/mg protein), and then letting react for 24h at RT 
in the dark.  Is this a reliable and efficient 

2.	If I try the CNBr digest on HSA without first 
reducing the disulfides, will the CNBr cleave or 
modify the disulfides?

3.	If I do reduce the disulfides of HSA but do 
not alkylate before the CNBr digest, will the 
cysteines end up alkylated (to the isothiocyanate)?

Any help would be appreciated!


More information about the Proteins mailing list