Cyanogen Bromide Digest Question
dmb618 at enh.nist.gov
Fri Mar 31 10:20:32 EST 1995
I am planning on doing a cyanogen bromide digest of
huamn serum albumin next week and I was looking for
some pointers from anyone with CNBr digest experience.
1. The method I found calls for dissolving the
protein in 70% formic acid, adding solid CNBr (2 mg
CNBr/mg protein), and then letting react for 24h at RT
in the dark. Is this a reliable and efficient
2. If I try the CNBr digest on HSA without first
reducing the disulfides, will the CNBr cleave or
modify the disulfides?
3. If I do reduce the disulfides of HSA but do
not alkylate before the CNBr digest, will the
cysteines end up alkylated (to the isothiocyanate)?
Any help would be appreciated!
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