CNBr -Sepharose 4B
lsc at novo.dk
Wed May 3 09:13:54 EST 1995
Why should urea or Gu interfere with your immobilization?
Neither contains "real" amino groups, i.e. amino groups which
would block CNBr activated Sepharose.
<Unknown> (anonymous) wrote:
> I am looking for some help in coupling a poorly soluble protein to CNBr-
> activated Sepharose 4B. We want to affinity purify some antibodies, but
> the antigen (a recombinant protein) is only soluble in guanidine HCl or
> urea. When diluted at least 20 fold with phosphate, the protein remains in
> solution, but at these concentrations the coupling efficiency will be very low.
> I was wondering if anyone has any experience of coupling a protein as a
> slurry, perhaps in the presence of a non-ionic detergent. Alternatively,
> is there an affinity matrix which is compatable with solvents containing
> amino groups.
> Thanks in advance
> Pete Jenner
> Dept of Mycobacterial Res.
More information about the Proteins