Kinase activated by Glycerol
maucuer at bisance.citi2.fr
Fri May 5 11:43:26 EST 1995
I produced a GST-Kinase fusion protein. When i tested its
autophosphorylation after elution with glutathione and
dialysis against kinase buffer, i observed a dramatic
enhancememt by adding 25% glycerol in the reaction.
Does anyone has experience about such effect ?
The GST-Kinase was contaminated by seemingly DnaK which
also seems to autophosphorylate.
Does anyone know about substrate(s) and specificity of
phosphorylation by DnaK ?
INSERM U153 phone (33) 1 43 37 17 23
17, rue du Fer a Moulin, 75005 Paris, France email maucuer at citi2.fr
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