Weird SDS-PAGE... HELP!!

Darren Fast fast at biochem.wisc.edu
Mon May 8 20:13:09 EST 1995


In article <95128.132452JPS144 at psuvm.psu.edu>, Joe Stains
<JPS144 at psuvm.psu.edu> wrote:

> Someone in my lab is running SDS-PAGE on a cell lysate.  However, when
> they run the gel, the sample lanes spread out (longitudinally), pushing
> the adjacent lanes out as well.  No one seems to have seen anything like
> this before.  We've tried several things to reduce salt, getting rid of
> any lysis buffer, decreasing sample load volume, but nothing seems to
> work.  We've even decreased the gel speed to hopefully correct the
> problem.
> 


I believe you may have missed the obvious.  Have you tried loading less
protein on the gel??

Darren

-- 
Darren Fast
Dept of Biochemistry
University of Wisconsin-Madison
ph. (608)-262-4705
fax (608)-262-3453
e-mail FAST at biochem.wisc.edu



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