Weird SDS-PAGE... HELP!!

Darren Fast fast at
Mon May 8 20:13:09 EST 1995

In article <95128.132452JPS144 at>, Joe Stains
<JPS144 at> wrote:

> Someone in my lab is running SDS-PAGE on a cell lysate.  However, when
> they run the gel, the sample lanes spread out (longitudinally), pushing
> the adjacent lanes out as well.  No one seems to have seen anything like
> this before.  We've tried several things to reduce salt, getting rid of
> any lysis buffer, decreasing sample load volume, but nothing seems to
> work.  We've even decreased the gel speed to hopefully correct the
> problem.

I believe you may have missed the obvious.  Have you tried loading less
protein on the gel??


Darren Fast
Dept of Biochemistry
University of Wisconsin-Madison
ph. (608)-262-4705
fax (608)-262-3453
e-mail FAST at

More information about the Proteins mailing list