Membrane preparations

Warren Wakarchuk Wakarchu at biologysx.lan.nrc.ca
Thu May 11 22:10:45 EST 1995


In article <D8FqoA.A8s at cc.umontreal.ca> parents at ERE.UMontreal.CA (Parent Stephane) writes:
>From: parents at ERE.UMontreal.CA (Parent Stephane)
>Subject: Membrane preparations
>Date: Thu, 11 May 1995 22:33:44 GMT

>Preparation of crude membrane fraction is used routinely in many 
>laboratories studying membrane proteins. The centrifugal force used to 
>sediment this crude particulate fraction which include the plasma 
>membranes vary from an investigator to the next. A rapid survey indicates 
>that it varies from 10,000g to 60,000g with 45,000g being used very 
>often. Is there any rationale for these differences? Could anybody 
>recommand a refference which would be useful to determine the appropriate 
>centrifugal force to use ?   

>Michel Bouvier ;Bouvier at BCH.UMontreal.ca


 Many investigators consider a protein to be soluble if it stays in solution 
after 100,000 X g for 1h.  We always make membranes by taking the pellet 
from a 100,000 X g spin (We use R avg = 100,000 ).

Warren Wakarchuk              Email:  wakarchu at biologysx.lan.nrc.ca
Immunochemistry                       warren at nrcbs8.bio.nrc.ca
IBS-NRCC                        
Ottawa, Ont.  CANADA
**Any opinions stated here are my own, not NRC's**



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