How much phosphate allowed in CD studies?

Matt Parker at332 at FreeNet.Carleton.CA
Thu May 11 07:33:59 EST 1995


	Hi! We are planning to do some far-UV CD work with our proteins.
We would like to use 100 mM potassium phosphate as the buffer. Does
anybody know whether this concetration of phosphate will interfere with
measurements at the low wavelength end? I'm not worried about 222 nm,
where we will be doing most of our measurements, but we'd like to be able
to get complete scans (down to 190 or so) of the proteins as well. 

	I've seen many papers where people used 50mM phosphate without
difficulty.

	Thanks!
		Matthew Parker




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