Western Blotting vs ELISA
hoekstra at unixg.ubc.ca
Fri May 12 12:00:29 EST 1995
Currently, I'm running a number of SDS-PAGE's, tranferring to
nitrocellulose, and immunoblotting for protein of interest (stress
I understand that western blotting gives more of a representation of
proteins presence/non-presence and was wondering if investigators
ROUTINELY run ELISA's to quantify (i.e. get a numerical value for
comparison) the protein of interest, as compared to a control sample?
In my mind, there is no doubt that the protein is present...are ELISA's
necessary then in addition? or is something like using a densitometer to
compare present bands sufficient?
PS: If anyone is personally researching stress proteins (ie HSP 70 etc.)
I would appreciate your reply..
\|||||/ Ken Hoekstra, Veterinary Technician, B.Sc.
O O University of British Columbia
o Graduate Student
\_/ Ken.Hoekstra at deepcove.com\\\///hoekstra at unixg.ubc.ca
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