quantitative immunoassays

Fiberman william at neuro.usc.edu
Mon May 15 20:19:38 EST 1995


Hello, netter,

I wish to detect changes in the level of a specific protein expressed in
brain tissue.  I have an antibody that will recognize the protein.  What is
the best assay I can run to quantitate the change in expression?  I don't need
absolute numbers, but I need an assay that will accurately detect changes in
protein levels.  I have tried western blots, but I feel this assay has a lot
of room for variation.  I would appreciate any suggestions.

-fm




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