RNeubig at umich.edu
Tue May 16 08:17:35 EST 1995
There are many strategies to doing this. You can have peptides
made on a large carrier (MAP) peptides for which you don't need
to add a carrier protein later. Talk to your peptide synthesis
facility about this.
Keyhole limpet hemocyanin is often used as a carrier as it isn't present in
mammalian systems so that you wouldn't get background staining
(eg of albumin if you use BSA as carrier).
Also, there are numerous companies that will actually make the
peptide and do your immunizations for you. Having done them
in our lab a number of years ago, I'd say that it is probably
worth the $ to have someone else do them and just send you the
serum. I don't have company names available but if you look
in any recent science or Nature mag I think you'd find them.
Rick Neubig RNeubig at umich.edu
University of Michigan Phone (313) 763-3650
http://www.umich.edu/~rneubig FAX (313) 763-4450
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