ThioFusion Expression System.

Bingjie Miao bmiao at students.wisc.edu
Wed May 17 20:20:25 EST 1995


Hi. I'm wondering whether anyone tried the ThioFusion Expression System
from Invitrogen. Supposely it could improve the solubility of your
protein. Also it can be purified by an affinity resin. All and all it
sounds pretty good. Have you had good luck with it? What's the typical
yield you can get? How easy it is to purify the fusion protein? Is there a
way of getting rid of the thioredoxin part? Would you recommend it? Any
comments and suggestions are greatly appreciated.
Thanks in advance.

Bingjie Miao
University of Wisconsin-Madison
bmiao at macc.wisc.edu



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