Removal of SDS from protein for structural studies

Dr. Jeff Lightfoot jlight at RESUNIX.RI.SICKKIDS.ON.CA
Thu May 18 20:19:52 EST 1995

What methods are recommended for removing 1% SDS from protein?   Is
dialyzing sufficient?  The protein is to be used for crystallography and/or
nmr.  Pierce sells a gel that is supposed to remove detergents, but they
keep bumping the availability date back.  Thanks for your help.


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