Removal of SDS from protein
Stephen P. Driska PhD
driska at astro.ocis.temple.edu
Fri May 19 17:59:29 EST 1995
Electroeluting a protein from a gel, with a buffer containing SDS
will give you a solution with protein and SDS. You can freeze dry this
in a small tube, and then rinse/wash it a few times with 90% ethanol. This
gets SDS, coomassie-blue, dithiothreitol, and even tris buffer I think, out
of your protein.
Of course its dead, denatured, and spread all over the walls of your
tube in a film. But that's the way you want it if your'e doing vapor phase
HCl hydrolysis for amino acid analysis (PicoTag).
Maybe this is useful for what you're doing.... Good Luck
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