fractionation of CHO cell membranes

Thomas Earnest TNEarnest at lbl.gov
Tue May 23 14:52:26 EST 1995


In order to fractionate CHO cells and seperate their 
membranes, many researchers have used the method of
nitrogen cavitation [pressurizing with nitrogen, then
release of the cells leading to cell bursting upon
sudden depressurization]. At some pressures the cell membranes
are broken but not subcellular organelles.
Unfortunately I have not found a commercial device for
this which is of adequate volume. 

Does anyone know of a vendor for a large "bomb" [yes, that's
what they're called] for this procedure? 
Any suggestions about other methods for this.

Please post or e-mail suggestions.

Thomas Earnest
Structural Biology Division
Lawrence Berkeley Laboratory

earnest at xtalu.lbl.gov



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