gel shift using hot protein as a probe?
hroychow at NMSU.EDU
Wed May 31 15:11:43 EST 1995
On 30 May 1995, Aron B. Jaffe wrote:
> I would like to look at protein-protein interactions on a non-
> denaturing gel using an 35S-labeled protein as my probe. Can I
> do this? Does anyone know of a reference?
Surely, if you have the specific mRNA for the protein that you want to
label you could simply do in vitro translation (using 35S-met), purify
the protein of your interest and use it as the probe. However, labeling
the pure protein with 125I may be a better and easier alternative.
Iodination protocols can be had from any protein protocol book or from
the Meth. Enzymol. volumes. Protein-protein crosslinking is achieved by
uv-irradiation, and this needs to be standardized for each system and the
Hiranya S. Roychowdhury
Plant Genetic Engineering Lab.
Box 3GL, NM State Univ.
Las Cruces, NM 88003
Phone: (505) 646-5785
hroychow at nmsu.edu
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