PAGE: Run times and voltages

Venkateswara rao Mulpuri vmulpuri at uoguelph.ca
Thu Nov 2 15:36:42 EST 1995


Nick Moody (nicholas.moody at jcu.edu.au) wrote:
: It is standard practise in our Department to run SDS-PAGE gels at 200V 
: until the dye front reaches the end of the gel (usually 45 miuntes). In 
: other papers I have read they use lower voltages for longer times (eg 40V 
: for 3 to 4 hours). Can anyone tell me if there are any advantages in 
: using lower voltages and longer times. I use 10% discontinuous SDS=PAGE 
: gels and need to silver stain them.

: Thanks

: -- 

: Nicholas Moody
: Department of Biomedical and Tropical Veterinary Sciences
: James Cook University
: Townsville, Qld., 4811
: Australia

: Tel: +61 077 814 631
: Fax: +61 077 796 371





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