stripping Westerns

[Cornelius Krasel]

Seth Crosby (crosby at milbrandt.wustl.edu) wrote:
> Is there a protocol for stripping antibodies off Western blots 
> (nitrocellulose)?  Couldn't find any in the Antibody Bible.

There is one in the leaflet that comes with the ECL kit. I think
it involves heating in 2-ME. Another possibility is to strip with
100 mM Glycine pH 2.5.

Disclaimer: I'm not much of a Western blotting expert.

--Cornelius.

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