folin assay

Hisham Ibrahim hisham at adm.agri.kagoshima-u.ac.jp
Mon Nov 6 23:17:08 EST 1995


In article <47gq6b$mkp at ra.cc.wwu.edu>, Andrea Powers <powers> wrote:

> Can anyone help me find the recipie for the folin developing agent used in the
> folin assay?  The stuff we have in the lab has been giving me a prcipitate, so
> I assume thta it's too strong, but I dont know what to dilute it to, or if I
> need to add anything else. The label says 1:1, and the only protocol I
can find
> was written for a lab class where the solutions were all made up by a TA.  I
> would be extremly grtefull for any help -  -

 Usually we do not prepare Folin reagent because it is commercially available
from "nacalai tesque" code No. 372-05. This concentrated reagent need to be
diluted 12 times before use (1:11) in Lowry method modified by Miller.
Best luck.

Hisham
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