Help with Hill Equn. needed
tday at netcom.com
Mon Nov 6 11:01:49 EST 1995
> >The data also fits Scatchard type eqn. which assumes one site, but not one
> >which assumes 2 sites or many. The question I still have in my mind is
> >whether this apparent 1site (or 1 type of non-interacting sites) could
> >really be a non-specific binding leading to inactivation.
> If the addition of a "ligand" destabilizes the protein, i.e. lowers the
> then that ligand must bind to the unfolded form. It seems reasonable
> would be a non-specific binding. Of course, one can always describe
> either site-specific or non-specific formalisms. Look at the work on urea and
> GuHCl denaturation.
Unfortunately, this protein unfolds irreversibly, and has several
unfolding transitions visible in DSC. So it is also possible that it
binds to a folded intermediate (or the fully folded form) and destabilises
that, rather than stabilising the unfolded form. This still begs the
question, if the binding were non-specific, wouldn't the data be a poor
fit to a n=1 binding equn.?
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