Help with Hill Equn. needed
Martin J. Gallagher
marty at ionchannel.med.harvard.edu
Tue Nov 7 13:07:55 EST 1995
In article <tday-0211952037420001 at tday.slip.netcom.com>, tday at netcom.com
(Tony Day) wrote:
> We have a protein which is destabilised by a small molecule. The effect
> saturates at low mM concentrations. The argument in the lab is whether it
> is non-specific or specific. I measured a series of inactivation rates at
> different inactivator concentrations. The data were fitted to a
> non-linear form of the Hill Eqn.. This gave a good fit with a Hill
> coefficient of 1.02. at a Kd of ~.8mM. Also the residuals looked random.
> However, I am a novice at using the Hill Equn.
> My question is as follows: Is there any other reasonable interpretation
> of the good fit and Hill coefficient of 1, than a specific single binding
> site (or identical sites)? In particular, could it plausibly be
> non-specific binding?
> Many thanks in advance.
> Tony Day
Can you get/synthesize a radioactive analog of your ligand?
Martin J. Gallagher phone: (617) 432-1729
Dept. of Neurobiology fax: (617) 734-7557
Harvard Medical School E-mail:marty at ionchannel.med.harvard.edu
220 Longwood Ave http://ionchannel.med.harvard.edu/~marty
Boston, MA 02115
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