non-block with PVDF ??

Stefan Baeckstroem seab
Sat Nov 11 18:32:55 EST 1995


Hello there.

Has anyone tested the hydrophobic non-block method with western when using PVDF
membranes published in a recent number of analytical biochemistry (sponsored by
millipore) ?

They say that you can save time, but it seems like it takes longer time to me.
After the blot you have to dry the membrane properly, which they recomend you
to do in rt overnight. Then you have earned two hours with the non-block, but
you lost one day having to wait for the membrane to dry before the
immunodetection.

Any comments ?
-- 
		Stefan Backstrom
		seab at nucleus.ibg.uu.se
		http://www.ibg.uu.se/~seab




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