ultrafiltration to v. small volume

Elias Klein e0klei01 at ulkyvm.louisville.edu
Mon Nov 13 11:32:53 EST 1995

Detergents form micellar solutions (colloidal) and present aggregates to 
the filtration medium.  Thus their effective Mol Wt is much greater than 
the mMW of the constituent detergent.  If you dialyze first through a 
non-adsorbing membrane  (cellulose, such as in the Pierce slidealyzer) 
you can reduce the detergent concentration.  

More information about the Proteins mailing list