Peptide Biotinylation

Michael Daws Michael.Daws at ucmp.umu.se
Mon Nov 13 10:41:32 EST 1995


I wish to immobilise a peptide on a column.  I think the easiest way to
do this would be to biotinylate the peptide at the C-terminus and link
it to streptavidin-agarose.
    a) Is this true?
    b) What is the easiest way to biotinylate specifically on the
       C-terminus of a peptide - do I link through a cysteine?
    c) How do I get rid of free biotin from a small peptide (approx.
       2kD)?






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