Protocol for proteases?

sujoy ghosh ghoshs at acpub.duke.edu
Sun Nov 19 11:47:03 EST 1995


I am trying to compare the stability of the wild-type and mutant variety 
of a protein by controlled proteolysis by, for example, trypsin.  Does 
anybody have a working protocol for trypsin treatment of proteins (such 
as time, concentration, stop conditions etc.). Thanks in advance.
Sujoy





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