GST fusion wont stick

cok at c.okane at
Thu Nov 23 08:12:37 EST 1995

A colleague of mine is trying to purify a GST fusion, but it won't bind to
glutathione beads. GST itself binds OK, so we think that it's something
about the fusion.

Does anyone have any suggestions? Would a GST antibody column be likely to
work instead, and if so is there anything like this available?

Cahir O'Kane
cok at

Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK
cok at

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