GST fusion wont stick

cok at mole.bio.cam.ac.uk c.okane at gen.cam.ac.uk
Thu Nov 23 08:12:37 EST 1995


Hi,
A colleague of mine is trying to purify a GST fusion, but it won't bind to
glutathione beads. GST itself binds OK, so we think that it's something
about the fusion.

Does anyone have any suggestions? Would a GST antibody column be likely to
work instead, and if so is there anything like this available?

Thanks,
Cahir O'Kane
cok at mole.bio.cam.ac.uk

-- 
Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK
cok at mole.bio.cam.ac.uk



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