Gel-elution

[Cornelius Krasel]

Hello Thomas :-)

fester at mpimg-berlin-dahlem.mpg.de wrote:
> I would like to elute protein complexes (mitochondrial respiratory
> complex III) from Blue Native Gels (about 7% acrylamide) and have
> no experience in doing so. I know electroelution is certainly the best
> way to go, unfortunately I don't have the necessary equipment.

AFAIK it is possible to electroelute proteins by putting the gel slice in
a dialysis bag and applying current. I've never done this, however...
(It would, nevertheless, circumvent the need for specialized equipment.)

--Cornelius.

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/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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