Gel-elution

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Tue Nov 28 04:10:52 EST 1995


Hello Thomas :-)

fester at mpimg-berlin-dahlem.mpg.de wrote:
> I would like to elute protein complexes (mitochondrial respiratory
> complex III) from Blue Native Gels (about 7% acrylamide) and have
> no experience in doing so. I know electroelution is certainly the best
> way to go, unfortunately I don't have the necessary equipment.

AFAIK it is possible to electroelute proteins by putting the gel slice in
a dialysis bag and applying current. I've never done this, however...
(It would, nevertheless, circumvent the need for specialized equipment.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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